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| Product Manual BactoReal® Kit Leptospira spp. Multiplex (16S rDNA+LipL32) |
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| Material Safety Data Sheet BactoReal® Kit Leptospira spp. Multiplex (16S rDNA+LipL32) |
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Background: The spirochaetal Leptospira genus consists of pathogenic species (P1) (L. interrogans, L. noguchii, L. weilii, L. kirschneri, L. alexanderi, L. borgpetersenii, L. santarosai, L. kmetyi, Leptospira genomospecies 1), of intermediately pathogenic species (P2) (L. inadai, L. fainei, L. broomii, L. licerasiae, L. wolffii) and of saprophytic (non- pathogenic) species (S) (L. biflexa, L. meyeri, L. wolbachii, Leptospira genomospecies 3, 4 and 5). Members of Leptospira can also be grouped into serovars. Currently over 200 serovars are recognized in the genus Leptospira and a few serovars are found in more than one species of Leptospira. In contrast to pathogenic Leptospira, Saprophytes can also grow outside the host animal. Pathogenic Leptospira species cause leptospirosis, a disease affecting many mammalian species, including humans. Animals may become unapparent carriers, and shedding of leptospires, primarily in the urine, serves as a source of infection for other animals and humans. The clinical signs associated with leptospirosis are variable and depend on the infecting serovar. Leptospira are present in the blood during the first 5 to 10 days after disease onset. Only pathogenic Leptospira species express an outer membrane lipoprotein encoded by the LipL32 gene.
Description: BactoReal® Kit Leptospira spp. Multiplex (16S rDNA+LipL32) uses multiplex real-time PCR for the detection and differentiation of the LipL32 gene of pathogenic (P1) Leptospira species as well as of the 16S rDNA gene of both pathogenic (P1) and intermediately pathogenic (P2) Leptospira species. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUB00753 | 50 | FAM, VIC | Cy5 | LipL32 & 16S rDNA |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: 16S rDNA and LipL32 gene of Leptospira spp.
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Leptospira spp. Multiplex (16S rDNA+LipL32) was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio moledcular systems), LightCycler® 480 II (Roche) and cobas z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5/6/7 (Thermo Fisher Scientific)).