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Product Manual BactoReal® Kit Haemophilus influenzae Download PDF
Material Safety Data Sheet BactoReal® Kit Haemophilus influenzae Download PDF
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Background:  The 16 species of Haemophilus are Gram-negative bacteria, occasionally colonizing the mucosa of humans and animals. Some Haemophilus species are the causative agents of various infections. For example, H. aegyptius causes purulent conjunctivitis, H. parainfluenzae is a rare cause of a form of endocarditis. Haemophilus influenzae can be found exclusively in the mucosa of humans, mainly in the upper respiratory tract. Transmission occurs through close contact with contaminated respiratory droplets. In sum, six capsular serotypes (a-f) can be distinguished, out of which type b (Hib) is the most prevalent and virulent one causing bloodstream infections and meningitis mainly in children less than 5 years. In contrast, unencapsulated strains (also called nontypeable H. influenzae, NTHi) of H. influenzae are part of the normal bacterial flora of humans and are occasionally pathogen. These strains can cause otitis, sinusitis, epiglottitis, bronchitis, pneumonia and less frequently meningitis and bacteraemia. The Omp P6 gene of H. influenzae is present in both the encapsulated and unencapsulated strains of H. influenzae.

Description:  BactoReal® Kit Haemophilus influenzae is a real-time PCR for the qualitative detection of the Omp P6 gene of Haemophilus influenzae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Haemophilus influenzae
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB05653 50 FAM Cy5 Omp P6 gene
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: Omp P6 gene of Haemophilus influenzae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Haemophilus influenzae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit is designed for the detection of Haemophilus influenzae DNA using real-time PCR, supporting the diagnosis of bacterial respiratory infections.
The kit amplifies a conserved region of the Haemophilus influenzae genome to ensure reliable identification of this pathogen.
Validated clinical samples include respiratory tract specimens such as nasopharyngeal swabs, throat swabs, bronchoalveolar lavage, and sputum.
Yes, the kit contains an internal control to monitor DNA extraction and to detect potential PCR inhibition.
Real-time PCR enables rapid, sensitive, and specific detection of Haemophilus influenzae, offering faster results compared to culture-based methods.