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| Product Manual BactoReal® Kit Chlamydophila psittaci/abortus |
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| Material Safety Data Sheet BactoReal® Kit Chlamydophila psittaci/abortus |
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Background: Chlamydophila psittaci (previously classified as Chlamydia psittaci) is an intracellular bacterium that may cause endemic avian chlamydiosis in feral birds and domesticated poultry, epizootic outbreaks in mammals such as cattle, pigs, sheep and horses, and respiratory psittacosis in humans (also known as parrot fever and ornithosis). It is transmitted by inhalation, contact or ingestion among birds and to mammals. Psittacosis in birds and in humans often starts with flu-like symptoms and becomes a life-threatening pneumonia. Chlamydophila psittaci is present in feces, nasal secretions, and feathers of infected birds. Chlamydophila abortus (previously classified as Chlamydia psittaci serotype 1) is one of the major infectious causes of abortion in sheep, cattle and goats worldwide by colonizing in the placenta. Enzootic abortion in sheep is also known as ovine enzootic abortion (OEA) or enzootic abortion of ewes (EAE). Infection with strains of this microorganism has also been associated with abortion and other clinical symptoms also in humans.
Description: BactoReal® Kit Chlamydophila psittaci/abortus is a real-time PCR for the qualitative detection of the omp1 gene of Chlamydophila psittaci and Chlamydophila abortus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUB03053 | 50 | FAM | Cy5 | omp1 gene |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: omp1 gene of Chlamydophila psittaci und Chlamydophila abortus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: BactoReal® Kit Chlamydophila psittaci/abortus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).