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Product Manual BactoReal® Kit Chlamydophila pneumoniae Download PDF
Material Safety Data Sheet BactoReal® Kit Chlamydophila pneumoniae Download PDF
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Background:  Chlamydophila pneumoniae (formerly Chlamydia pneumoniae) is a gram-negative obligate intracellular bacterium and has a global distribution. It was formerly thought to be a specific human pathogen, but in the meantime it has also been found in horses, koala, amphibians, reptiles and other animals. In humans, it commonly causes pharyngitis, bronchitis and atypical pneumonia (community-acquired pneumonia, CAP).

Description:  BactoReal® Kit Chlamydophila pneumoniae is a real-time PCR for the qualitative detection of the ompA gene of Chlamydophila pneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Chlamydophila pneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB00953 50 FAM Cy5 ompA gene
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ompA gene of Chlamydophila pneumoniae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Chlamydophila pneumoniae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit is designed for the molecular detection of Chlamydophila pneumoniae DNA using real-time PCR, supporting the diagnosis of respiratory tract infections.
The kit specifically amplifies a conserved region of the Chlamydophila pneumoniae genome to ensure reliable pathogen identification.
Validated sample types include respiratory specimens such as nasopharyngeal swabs, throat swabs, bronchoalveolar lavage fluid, and sputum.
Yes, the kit provides an internal control to monitor nucleic acid extraction efficiency and detect possible PCR inhibition.
Real-time PCR enables rapid, sensitive, and specific detection of Chlamydophila pneumoniae, overcoming the limitations of culture-based diagnostics.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.