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Product Manual BactoReal® Kit Brucella spp. Download PDF
Material Safety Data Sheet BactoReal® Kit Brucella spp. Download PDF
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Background:  Brucellosis - also called Bang''s disease, Crimean fever, Gibraltar fever, Malta fever, Maltese fever, Mediterranean fever, rock fever, or undulant fever - is a zoonotic infection of domesticated and wild animals, caused by bacteria of the genus Brucella. Brucella are gram-negative, intracellular bacteria that can infect many species of animals including wildlife species and marine mammals, as well as humans. Species infecting domestic livestock are B. melitensis (goats and sheep), B. suis (pigs), B. abortus (cattle and bison), B. ovis (sheep), and B. canis (dogs). Humans become infected by ingestion of food products of animal origin (such as undercooked meat or unpasteurized milk or dairy products), direct contact with infected animals, or inhalation of infectious aerosols. Transmission from human to human is rare but possible.

Description:  BactoReal® Kit Brucella spp. is a real-time PCR for the qualitative detection of the IS711 region of B. abortus, B. ovis, B. melitensis, B. suis and B. canis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Brucella spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB02153 50 FAM Cy5 IS711
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: IS711 region of Brucella spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Brucella spp. was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit enables the molecular detection of Brucella species DNA, supporting diagnosis of human brucellosis using real-time PCR technology.
The kit amplifies a Brucella-specific sequence within the bcsp31 gene, ensuring accurate and specific identification of Brucella spp.
Yes, the kit includes an internal control to verify extraction efficiency and to detect possible PCR inhibition.
Validated sample types include blood, serum, tissue, and other clinical specimens relevant for suspected brucellosis cases.
Rapid and sensitive detection of Brucella spp. is crucial for early diagnosis and treatment of brucellosis, a zoonotic disease that can cause chronic and systemic illness.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.