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Download All Background: Aspergillus is a fungal genus consisting of several hundred species. Aspergillus species are ubiquitously found and some species can cause opportunistic infections such as allergic bronchopulmonary aspergillosis, pulmonary aspergilloma and invasive aspergillosis. Invasive aspergillosis is increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. Aspergillus fumigatus is the predominant disease-causing species. However, other species than A. fumigatus, in particular A. terreus and A. flavus but also A. niger, A. nidulans and A. ustus have gained greater clinical significance. Not only the detection, but also the identification of the fungal species is important. The species might provide information on a potential contamination, colonisation or infection and moreover antifungal resistance.
Description: MycoReal® Kit Aspergillus is a real-time PCR test for the detection of Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Aspergillus niger and Aspergillus nidulans from samples purified from bronchoalveolar lavages (BAL), blood, aspirates, cerebrospinal fluid and tissue. Furthermore, it detects some other closely related Aspergillus/Emericella species belonging to the Aspergillus section Fumigati, Flavi, Terrei, Nigri, Nidulantes, Usti and Versicolori. The kit allows the detection and to some extent also the distinction of medically important Aspergillus species. Our specific probes (FAM and VIC labelled) detect the internal transcribed spacer 2 (ITS2-region, multicopy gene) of Aspergillus. Analysis is done with Assay Mix 1 and Assay Mix 2 in two parallel PCR-reactions. The internal DNA positive control (DNA IPC) is detected in the Cy5 channel and is used as DNA extraction as well as PCR inhibition control. MycoReal® Kit Aspergillus aides in the diagnosis of Aspergillus infections including invasive aspergillosis, supporting lifesaving early and targeted antifungal treatment of patients.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUF00253 | 50 | FAM, VIC | Cy5 | ITS2-region |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: ITS2-region of Aspergillus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR-platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: MycoReal® Kit Aspergillus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific) and cobas z 480 Analyzer (Roche), but is also suitable for other real-time PCR instruments.