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Product Manual ViroReal® Kit RSV (A & B) Download PDF
Material Safety Data Sheet ViroReal® Kit RSV (A & B) Download PDF
Declaration of Conformity ViroReal® Kit RSV (A & B) Download PDF
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Background:  Human respiratory syncytial virus (RSV) is a negative-sense, single-stranded RNA virus (ss (-)RNA) of the family Paramyxoviridae. RSV consists of two antigenic subtypes, A and B. It causes respiratory tract infections and is a major cause of lower respiratory tract infections and hospital visits during infancy and childhood. In addition, RSV is recognized as an important cause of respiratory illness in older adults. Infection is most common during the winter and early spring e.g. in the rainy season.

Description:  ViroReal® Kit RSV (A & B) is a real-time PCR for the qualitative detection of the nucleocapsid protein gene of the human respiratory syncytial virus (RSV) type A and B. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit RSV (A & B)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV02153 50 FAM Cy5 nucleocapsid protein gene
For in vitro diagnostic use
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: nucleocapsid protein gene of RSV (A & B)
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • Contains RNase Inhibitor to block RNA degradation
  • CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit RSV (A & B) was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit detects RNA of the nucleocapsid protein gene of human respiratory syncytial virus (RSV) lineages A and B using one-step reverse transcription real-time PCR with detection in the FAM channel.
RSV is a major cause of lower respiratory tract infections, bronchiolitis, and pneumonia in infants and young children, and it is also increasingly recognized as a significant pathogen in older adults. Rapid molecular detection supports early diagnosis and appropriate patient management.
Validated specimens include RNA extracts from human respiratory tract samples, particularly nasopharyngeal swabs. Stool samples may also be used under certain conditions.
An internal RNA positive control (IPC) is included, added during extraction, and detected in the Cy5 channel. It ensures extraction efficiency and identifies potential PCR inhibition.
The limit of detection (LoD95%) is 50.2 target copies per reaction for RSV A and 41.6 copies per reaction for RSV B. Diagnostic evaluation showed 100% sensitivity and 100% specificity.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.