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Download All Background: Human rhinovirus (HRV) is a single-stranded positive sense RNA virus (ss(+)RNA) in the genus Enterovirus of the family Picornaviridae. Rhinoviruses are the most common viral infective agents in humans and are the predominant cause of the common cold. RV infections are chiefly limited to the upper respiratory tract. Although infections occur year-round, the incidence is highest during fall and spring. Most cases are mild and self-limited. Rhinovirus C, unlike the A and B species, may be able to cause sometimes more severe infections.
Description: ViroReal® Kit Rhinovirus (A, B, C) is a real-time PCR for the qualitative detection of the 5’ UTR of the human rhinovirus species A, B and C. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUV01953 | 50 | FAM | Cy5 | 5’ UTR |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: 5’ UTR of Rhinovirus (A, B, C)
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: ViroReal® Kit Rhinovirus (A, B, C) was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).