Which genetic target is amplified with the ViroReal® Kit Parechovirus?

The kit detects RNA of the polyprotein gene of Human Parechovirus using one-step reverse transcription real-time PCR with probe detection in the FAM channel.

What diseases are associated with human parechoviruses?

Human parechoviruses are linked to gastrointestinal, respiratory, and central nervous system infections. Severe disease may occur in neonates and immunocompromised individuals.

Which clinical specimens are validated for this kit?

Validated samples include RNA extracts from serum, cerebrospinal fluid, nasal discharge, urine, and stool.

How does the kit monitor RNA extraction and PCR inhibition?

An internal RNA positive control (IPC) is added during sample extraction and detected in the Cy5 channel, ensuring RNA extraction efficiency and detecting potential RT-PCR inhibition.

What is the analytical sensitivity of the kit?

The analytical sensitivity is approximately 100 RNA copies per PCR, with linear detection from 100 to 1,000,000 copies per reaction.

ViroReal® Kit Parechovirus – RT-PCR Detection of Polyprotein Gene of Human Parechovirus (Strains 1–6)

Name: ViroReal® Kit Parechovirus – RT-PCR Detection of Polyprotein Gene of Human Parechovirus (Strains 1–6)
Brand: ingenetix GmbH
SKU: DHUV01753
Rating: 5.0 (1 reviews)

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Product Manual ViroReal^® Kit Parechovirus	Download PDF
Material Safety Data Sheet ViroReal^® Kit Parechovirus	Download PDF
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Background: Human parechoviruses are non-enveloped, positive single-stranded RNA viruses (ss(+)RNA) and are members of the Picornaviridae family. Six serotypes of human parechovirus have been identified (human parechovirus 1-6). Parechoviruses are responsible for gastrointestinal, respiratory and central nervous system infections. 95% of humans are carriers of human parechovirus since early life.

Description: ViroReal^® Kit Parechovirus is a real-time PCR for the qualitative detection of the polyprotein gene of human parechovirus strains 1-6. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal^® Kit Parechovirus

Order Number	Reactions	Channel Pathogen	Channel IPC	Target
DHUV01753	50	FAM	Cy5	polyprotein gene

For research use only
Shipping Temperature: -20 °C dry ice

Product Features:

Amplification and detection: polyprotein gene of Parechovirus
Transcription with thermostable MMLV Reverse Transcriptase
Contains RNase Inhibitor to block RNA degradation
Real-time PCR with rapid hot-start Taq DNA polymerase
ROX™ dye as passive reference
Internal Positive Control System to exclude false-negative results
Optimized to handle RT-PCR inhibitors
PCR platforms: runs on all established standard real-time PCR platforms
Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal^® Kit Parechovirus was validated with the ABI^® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P^® (Agilent), qTOWER³G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler^® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio^™ 5 / 6 / 7 (Thermo Fisher Scientific)).

BactoReal^® , MycoReal^®, ParoReal ^® and ViroReal^® Kits run with the same thermal cycling conditions.

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