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Product Description ViroReal® Kit Parainfluenzavirus Download PDF
Product Manual ViroReal® Kit Parainfluenzavirus Download PDF
Material Safety Data Sheet ViroReal® Kit Parainfluenzavirus Download PDF
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Background:  Human parainfluenzaviruses (HPIV) are enveloped, negative single-stranded RNA viruses (ss(-)RNA) of the family Paramyxoviridae. At present four serotypes are known (types 1, 2, 3, 4). Parainfluenzavirus type 1 and 3 belong to the genus Respirovirus, while parainfluenzavirus type 2 and 4 belong to the genus Rubulavirus. Human parainfluenzavirus type 1 (HPIV-1) is the major cause of acute croup, but can also cause respiratory tract infections at all ages. Human parainfluenzavirus type 2 (HPIV-2) can also cause croup as well as upper and lower respiratory tract illnesses. Human parainfluenzavirus type 3 (HPIV-3) causes bronchiolitis and pneumonia. Human parainfluenzavirus type 4 with the subtypes 4a and 4b is less likely to cause severe disease.

Description:  ViroReal® Kit Parainfluenzavirus uses RT real-time PCR for the detection of the hemagglutinin-neuraminidase glycoprotein gene (HN gene) of human parainfluenzavirus types 1, 2, 3 and 4 (HPIV-1, HPIV-2, HPIV-3, HPIV-4). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Parainfluenzavirus
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV01653 50 FAM, VIC Cy5 HN gene
For in vitro diagnostic use
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: HN gene of Parainfluenzavirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Parainfluenzavirus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit detects RNA of the hemagglutinin-neuraminidase (HN) gene of human parainfluenzavirus types 1–4. HPIV-1 and HPIV-3 are detected in the FAM channel, while HPIV-2 and HPIV-4 are detected in the VIC channel.
HPIVs are major causes of respiratory illnesses. HPIV-1 and HPIV-2 can cause croup, HPIV-3 is associated with bronchiolitis and pneumonia, while HPIV-4 is less common but can still contribute to respiratory disease. Rapid detection guides patient care and infection control.
The kit is validated for RNA extracts from human respiratory tract samples, especially nasopharyngeal swabs.
An internal RNA positive control (IPC), added during extraction and detected in the Cy5 channel, verifies RNA extraction efficiency and identifies possible RT-PCR inhibition.
The LoD95% is 47 target copies/reaction for HPIV-1, 335 copies for HPIV-2, 294 copies for HPIV-3, and 346 copies for HPIV-4. Diagnostic evaluation showed 100% sensitivity and 100% specificity in tested samples.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.