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Product Manual ViroReal® Kit Human Coronavirus beta (HKU1, OC43 & MERS) Download PDF
Material Safety Data Sheet ViroReal® Kit Human Coronavirus beta (HKU1, OC43 & MERS) Download PDF
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Background:  Coronaviruses are positive single-stranded (+)ssRNA RNA viruses of the family Coronaviridae. Several different strains of coronaviruses are currently known to infect humans (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV, NcoV and HCoV-EMC). Strains HCoV-229E, HCoV-NL63, HCoV-OC43, MERS-CoV and HCoV-HKU1 cause cold, upper respiratory infection, bronchiolitis and pneumonia in humans. SARS-CoV causes the Severe Acute Respiratory Syndrome.

Description:  ViroReal® Kit Human Coronavirus beta is a real-time PCR for the qualitative detection of the N gene of human coronavirus OC43, HKU1 and MERS. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Human Coronavirus beta (HKU1, OC43 & MERS)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV01253 50 FAM, VIC Cy5 N gene
For research use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of Human Coronavirus beta
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Human Coronavirus beta was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit detects RNA of the N gene of Human Coronavirus beta HKU1 and OC43 in the FAM channel, and the N gene of Human Coronavirus beta MERS in the VIC channel using multiplex RT-PCR.
These coronaviruses are associated with respiratory infections ranging from mild colds to severe pneumonia. MERS-CoV in particular can cause severe illness with high mortality. Rapid detection enables early diagnosis and clinical management.
The kit is validated for RNA extracts from respiratory tract samples, including nasopharyngeal swabs and aspirates, and cerebrospinal fluid.
An internal RNA positive control (IPC) is added during extraction and detected in the Cy5 channel. It monitors RNA extraction efficiency and detects possible RT-PCR inhibition.
The kit has been validated on multiple PCR platforms (ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio, etc.). Analytical specificity is ensured by highly specific primers and probes. LoD95% values are assay-dependent, and the kit demonstrated 100% specificity in validation.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.