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Product Manual ViroReal® Kit Human Coronavirus alpha (NL63 & 229E) Download PDF
Material Safety Data Sheet ViroReal® Kit Human Coronavirus alpha (NL63 & 229E) Download PDF
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Background:  Coronaviruses are positive single-stranded (+)ssRNA RNA viruses of the family Coronaviridae. Several different strains of coronaviruses are currently known to infect humans (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV, NcoV and HCoV-EMC). Strains HCoV-229E, HCoV-NL63, HCoV-OC43, MERS-CoV and HCoV-HKU1 cause cold, upper respiratory infection, bronchiolitis and pneumonia in humans. SARS-CoV causes the Severe Acute Respiratory Syndrome.

Description:  ViroReal® Kit Human Coronavirus alpha is a real-time PCR for the qualitative detection of the N gene of human coronavirus NL63 and 229E. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Human Coronavirus alpha (NL63 & 229E)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV01153 50 FAM, VIC Cy5 N gene
For research use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of Human Coronavirus alpha
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Human Coronavirus alpha was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit detects RNA of the N gene from Human Coronavirus alpha NL63 and 229E using real-time RT-PCR.
NL63 is detected in the FAM channel and 229E in the VIC channel, allowing distinct identification of both viruses.
The internal RNA positive control (IPC) monitors RNA extraction efficiency and detects possible RT-PCR inhibition in the Cy5 channel.
RNA extracts from respiratory tract samples such as nasal and throat swabs are recommended.
The kit targets the N gene of both HCoV alpha NL63 and HCoV alpha 229E.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.