Which pathogens are detected with the BactoReal® Kit P. aeruginosa & Enterobacter spp.?

The kit detects the 16S rRNA gene of Pseudomonas aeruginosa in the FAM channel and the rpoB gene of Enterobacter species in the VIC channel using multiplex real-time PCR.

Why is Pseudomonas aeruginosa an important clinical target?

P. aeruginosa is a major cause of severe hospital-acquired infections such as pneumonia, bloodstream infections, and wound infections, especially in immunocompromised and burn patients. Many strains are multidrug resistant, making rapid detection critical.

Which Enterobacter species can be identified with this kit?

The kit detects DNA from Enterobacter cloacae complex members (e.g., E. cloacae, E. hormaechei, E. ludwigii, E. roggenkampii), E. asburiae, E. bugandensis, E. huaxiensis, E. sichuanensis, and others, as well as some Klebsiella and Citrobacter species. Enterobacter kobei is detected with lower sensitivity.

Which clinical samples are validated for use with this kit?

Validated specimens include EDTA blood, cerebrospinal fluid, aspirates, and biopsies.

How does the kit ensure reliable results?

The kit contains an internal DNA positive control (IPC), added during extraction and detected in the Cy5 channel, which monitors DNA extraction efficiency and excludes PCR inhibition.

BactoReal® Kit P. aeruginosa & Enterobacter spp. – Real-Time PCR Detection of 16S rRNA Gene of Pseudomonas aeruginosa and rpoB Gene of Enterobacter spp.

Name: BactoReal® Kit P. aeruginosa & Enterobacter spp. – Real-Time PCR Detection of 16S rRNA Gene of Pseudomonas aeruginosa and rpoB Gene of Enterobacter spp.
Brand: ingenetix GmbH
SKU: DHUS00553
Rating: 5.0 (1 reviews)

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Product Manual BactoReal^® Kit P. aeruginosa & Enterobacter spp.	Download PDF
Material Safety Data Sheet BactoReal^® Kit P. aeruginosa & Enterobacter spp.	Download PDF
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Background: Pseudomonas spp. are Gram-negative rod bacteria found in soil, water and plants. In healthy individuals, they do not cause infection, but immunocompromised people can be infected. Pseudomonas aeruginosa as well as other Pseudomonas species such as P. paucimobilis, P. putida, P. fluorescens or P. acidovorans cause nosocomial and antibiotic-resistant infections which are difficult to treat and can be life-threatening. Pseudomonas infections can lead to sepsis, with P. aeruginosa being the most common species. Pseudomonas bacteremia is indicative of contaminated infusion solutions, medications or disinfectants used in the placement of intravenous catheters. Pseudomonas aeruginosa is also responsible for wound infection and bacteremia in burn patients, with high rates of multidrug resistance. Enterobacter species are Gram-negative bacteria causing a wide variety of nosocomial infections, including those affecting the lungs, urinary tract, intrabdominal cavity and intravascular devices. Enterobacter asburiae can be detected in urine, stool and blood samples. E. bugandensis can cause neonatal sepsis. E. cloacae can lead to wound infections and catheter-associated urinary tract infections and is often resistant to antibiotics. E. ludwigii and E. roggenkampii belong to the Enterobacter cloacae complex. Enterobacter huaxiensis, E. chuandaensis and E. chengduensis were discovered in the last few years in China and can cause sepsis. E. oligotrophica is an oligotrophic bacterium which has been recently isolated in Japan. E. sichuanensis was isolated in 2016 from a urine sample taken from a patient in China.

Description: BactoReal^® Kit P. aeruginosa & Enterobacter spp. is a real-time PCR for the qualitative detection of the DNA of Pseudomonas aeruginosa (16S rRNA gene) and Enterobacter spp. (rpoB gene). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal^® Kit P. aeruginosa & Enterobacter spp.

Order Number	Reactions	Channel Pathogen	Channel IPC	Target
DHUS00553	50	FAM, VIC	Cy5	16S rRNA + rpoB

For in vitro diagnostic use
Shipping Temperature: -20 °C to +4 °C

Product Features:

Amplification and detection: 16S rRNA gene of P. aeruginosa and the rpoB gene of Enterobacter species
Real-time PCR with rapid hot-start Taq DNA polymerase
ROX™ dye as passive reference
Internal Positive Control System to exclude false-negative results
Optimized to handle PCR inhibitors
PCR platforms: runs on all established standard real-time PCR platforms
Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal^® Kit P. aeruginosa & Enterobacter spp. was validated with the ABI^® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P^® (Agilent), qTOWER³G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler^® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio^™ 5 / 6 / 7 (Thermo Fisher Scientific)).

BactoReal^® , MycoReal^®, PanReal, ParoReal ^® and ViroReal^® Kits run with the same thermal cycling conditions.

BactoReal^® Kit P. aeruginosa and Enterobacter spp.

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