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Download All Background: Staphylococcus aureus is a Gram-positive bacterium, which can be found on skin and in mucous membranes. This bacterium can cause pneumonia, meningitis and sepsis and is a microorganism responsible for nosocomial infections. Some strains are resistant to antibiotics like methicillin and vancomycin and therefore more difficult to treat. Staphylococcus epidermidis is a Gram-positive bacterium colonizing skin and mucous membranes of humans. It can lead to infections through various implanted medical devices such as joint prostheses, cardiovascular devices or heart valves. Staphylococcus saprophyticus can lead to urinary tract infections, with young women being more severely affected. Other species of staphylococci such as S. lugdunensis, S. haemolyticus, S. warneri, S. schleiferi, S. intermedius are generally non-pathogenic but can lead to infections in rare cases.
Description: BactoReal® Kit Staphylococcus & S. aureus is a real-time PCR for the qualitative detection of the Staphylococcus aureus (nuc gene) and Staphylococcus spp. (23S rRNA gene). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUS00253 | 50 | FAM, VIC | Cy5 | nuc + 23S rDNA |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: nuc gene of Staphylococcus aureus and 23S rRNA gene of Staphylococcus spp.
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Staphylococcus spp. & S. aureus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).