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Download All Background: Escherichia coli is a Gram-negative bacterium inhabiting the normal intestinal flora. Most E. coli strains are non-pathogenic but some strains of this species can cause disease such as urinary tract infections, gastrointestinal infections, or sepsis. Klebsiella species are Gram-negative, facultatively anaerobic bacteria found ubiquitously in the environment. They are part of the healthy gut flora in humans. Outside the intestine they can lead to infections such as pneumonia and sepsis. Some strains show antibiotic resistance and are therefore difficult to treat. Both E. coli and Klebsiella are microorganisms responsible for nosocomial infections.
Description: BactoReal® Kit E. coli & Klebsiella is a real-time PCR for the qualitative detection of the Escherichia coli (dxs gene), Klebsiella pneumoniae, K. quasipneumoniae, K. variicola, K. oxytoca and K. aerogenes (khe gene). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUS00153 | 50 | FAM, VIC | Cy5 | dxs + khe |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: dxs gene of Escherichia coli and khe gene of Klebsiella pneumoniae, K. quasipneumoniae, K. variicola, K. oxytoca and K. aerogenes
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit E. coli & Klebsiella was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).