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| Product Manual PanReal Kit Fungi & Bacteria |
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| Material Safety Data Sheet PanReal Kit Fungi & Bacteria |
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Background: Fungal diseases are mostly opportunistic infections with a great variety of common and uncommon pathogens. Up to 150 fungal species have been shown as potential human pathogens involving all body sites. Invasive mycoses are increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. These infections are mainly caused by yeasts and moulds. The mortality rate of invasive fungal infections is 40-100%. A rapid diagnosis improves the outcome. Non-invasive fungal infections (such as infections of the urogenitary tract, of the eye, of the skin, etc.) are mainly caused by Candida, Aspergillus, Acremonium, Fusarium and dermatophytes. Concerning bacterial infections, broad‐range PCR targeting the 16S rDNA gene is useful when antimicrobial therapy has already been initiated or when culture remains negative due to infections caused by bacteria with unusual growth requirements.
Description: PanReal Kit Fungi & Bacteria is a real-time PCR for the qualitative detection of the of fungi and bacteria in samples purified from bronchoalveolar lavages (BAL), aspirates, cerebrospinal fluid, tissue, paraffin embedded tissue and fungal/bacterial colonies. Due to contamination, PanReal Kit Fungi & Bacteria is less sensitive compared to species-specific assays. The kit is suitable only to a limited extent for the detection of fungi in blood samples. For phylogenetic identification of the pathogen, the PCR product has to be sequenced and analysed by BLAST. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUFB0153 | 50 | FAM, VIC | Cy5 | ITS region/16S rDNA |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: ITS2 region of fungi and 16S rDNA of bacteria
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: PanReal KitFungi & Bacteria was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche), CFX Dx (Bio-Rad) and T-COR 8 (Tetracore) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).