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Product Manual BactoReal® Kit A. baumannii & P. mirabilis Download PDF
Material Safety Data Sheet BactoReal® Kit A. baumannii & P. mirabilis Download PDF
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Background:  Acinetobacter baumannii is a Gram-negative, aerobic bacterium which is responsible for nosocomial infections. It occurs mainly in intensive care units. This bacterium is often resistant to antibiotics and thus difficult to treat. It can lead to pneumonia, catheter-associated infections and can also cause sepsis. Proteus mirabilis is a Gram-negative, facultatively anaerobic rod-shaped bacterium which frequently occurs in the large intestine and is generally non-pathogenic. However, in people with a weakened immune system, it can lead to urinary tract infections, wound infections, pneumonia and sepsis.

Description:  BactoReal® Kit A. baumannii & P. mirabilisis a real-time PCR for the qualitative detection of the Acinetobacter baumannii (rpoB gene) and Proteus mirabilis (ureC gene) from samples purified from human EDTA blood, aspirates and CSF. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit A. baumannii & P. mirabilis
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB05153 50 FAM, VIC Cy5 rpoB + ureC
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: rpoB gene of Acinetobacter baumannii and ureC gene of Proteus mirabilis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit A. baumannii & P. mirabilis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit detects the rpoB gene of Acinetobacter baumannii in the FAM channel and the ureC gene of Proteus mirabilis in the VIC channel using multiplex real-time PCR.
A. baumannii is a major cause of hospital-acquired infections such as pneumonia, catheter-associated infections, and sepsis, particularly in intensive care units. Its frequent multidrug resistance makes rapid molecular detection essential.
P. mirabilis is usually part of the intestinal flora but can cause urinary tract infections, wound infections, pneumonia, and sepsis in immunocompromised patients. Identifying its DNA supports early targeted therapy.
Validated specimens include EDTA blood, cerebrospinal fluid (CSF), and aspirates.
The kit includes an internal DNA positive control (IPC), added during DNA extraction and detected in the Cy5 channel. It monitors extraction efficiency and rules out PCR inhibition.