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Download All Background: Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. The genus Borrelia consists of numerous species. Besides the species belonging to the B. burgdorferi sensu lato-complex there are also human pathogenic Borrelia species causing relapsing fever (B. recurrentis, B. duttonii and B. hermsii). Species belonging to the B. burgdorferi sensu lato-complex and causing Lyme disease (Lyme borreliosis) are Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae, B. bavariensis, B. bissettii and B. spielmanii. These are associated with different genospecies-specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.
Description: BactoReal® Kit Borrelia burgdorferi sensu lato is a real-time PCR for the qualitative detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato (s.l.) complex. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUB05053 | 50 | FAM | Cy5 | ospA gene |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: ospA gene of Borrelia burgdorferi sensu lato
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: BactoReal® Kit Borrelia burgdorferi sensu lato was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche), cobas Z 480 Analyzer (Roche), CFX Opus Real-time PCR System (Bio-Rad), CFX Dx (Bio-Rad) and T-COR 8 (Tetracore). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).