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Product Manual BactoReal® Kit Streptococcus agalactiae Download PDF
Material Safety Data Sheet BactoReal® Kit Streptococcus agalactiae Download PDF
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Background:  Streptococcus agalactiae (also known as Group B streptococcus or GBS) is a beta-hemolytic Gram-positive streptococcus. It is pathogenic primarily for animals, but can also infect humans. Since S. agalactiae strains isolated from animals can be differed from those isolated from humans, transmission from animals to humans is rare. Streptococcus agalactiae is a member of the gastrointestinal normal flora in some humans and can spread to secondary sites including the vagina. For this reason, it can cause sepsis, pneumonia and meningitis in newborns. In persons with a weak immune system, it can cause infections of the urinary tract and of wounds, meningitis and sepsis. Streptococcus agalactiae secretes a protein (CAMP factor) which causes haemolysis in presence of the beta-toxin of Staphylococcus aureus in blood agar.

Description:  BactoReal® Kit Streptococcus agalactiae is a real-time PCR for the qualitative detection of the cfb gene of Streptococcus agalactiae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus agalactiae
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB01553 50 FAM Cy5 cfb gene
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: cfb gene of Streptococcus agalactiae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Streptococcus agalactiae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit is designed for the molecular detection of Streptococcus agalactiae (Group B Streptococcus), which is a major cause of neonatal sepsis and meningitis.
The kit amplifies the cfb gene, which is specific to Streptococcus agalactiae, ensuring reliable identification of the pathogen.
Yes, an internal control is included to monitor PCR performance and to detect potential inhibition in the reaction.
Validated sample types include vaginal and rectal swabs as well as other clinical specimens relevant for prenatal screening and infection diagnostics.
Rapid detection of Streptococcus agalactiae is crucial for preventing severe neonatal infections and guiding appropriate antibiotic prophylaxis during pregnancy.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.