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Product Manual BactoReal® Kit Listeria monocytogenes Download PDF
Material Safety Data Sheet BactoReal® Kit Listeria monocytogenes Download PDF
Declaration of Conformity BactoReal® Kit Listeria monocytogenes Download PDF
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Background:  Listeriosis is an infection caused by the consumption of contaminated food (milk, cheeses, ice cream, vegetables, sausages, meats and fish). Although there are other types of Listeria (L. ivanovii, L. innocua, L. welshimeri, L. seegligeri and L. grayi), the culprit pathogen for most listeriosis cases is L. monocytogenes. Gastrointestinal symptoms may remain the only symptoms or precede more severe clinical manifestations such as sepsis and meningitis. People at risk include the elderly population, immunosuppressed patients and foetuses of pregnant women. L. monocytogenes is a gram positive, facultative anaerobic, and facultative intracellular bacterium with an incubation time of at least seven days. It expresses a Beta hemolysin (hlyA gene) which causes destruction of red blood cells and has the ability to target other cells. The ability of L. monocytogenes to grow at temperatures as low as 0°C permits multiplication in refrigerated foods. L. monocytogenes is killed by pasteurization and cooking.

Description:  BactoReal® Kit Listeria monocytogenes is a real-time PCR for the qualitative detection of the hlyA gene of Listeria monocytogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Listeria monocytogenes
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB00853 50 FAM Cy5 hlyA gene
For in vitro diagnostic use
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: hlyA gene of Listeria monocytogenes
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Listeria monocytogenes was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit enables the detection of Listeria monocytogenes DNA using real-time PCR for reliable identification of this pathogen.
The kit amplifies the hlyA gene, which encodes listeriolysin O and is specific for Listeria monocytogenes.
Typical sample types include food products, environmental swabs, and clinical material relevant for identifying Listeria monocytogenes contamination or infection.
Yes, an internal control is included to verify nucleic acid extraction and to detect potential PCR inhibition.
Real-time PCR offers high sensitivity and specificity, enabling rapid and accurate detection of Listeria monocytogenes compared to conventional culture methods.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.