Back
Inquiry

Download

Product Manual BactoReal® Kit Leptospira spp. (LipL32) Download PDF
Material Safety Data Sheet BactoReal® Kit Leptospira spp. (LipL32) Download PDF
Download All

Background:  The spirochaetal Leptospira genus consists of pathogenic species (P1) (L. interrogans, L. noguchii, L. weilii, L. kirschneri, L. alexanderi, L. borgpetersenii, L. santarosai, L. kmetyi, Leptospira genomospecies 1), of intermediately pathogenic species (P2) (L. inadai, L. fainei, L. broomii, L. licerasiae, L. wolffii) and of saprophytic (non- pathogenic) species (S) (L. biflexa, L. meyeri, L. wolbachii, Leptospira genomospecies 3, 4 and 5). Members of Leptospira can also be grouped into serovars. Currently over 200 serovars are recognized in the genus Leptospira and a few serovars are found in more than one species of Leptospira. In contrast to pathogenic Leptospira, Saprophytes can also grow outside the host animal. Pathogenic Leptospira species cause leptospirosis, a disease affecting many mammalian species, including humans. Animals may become unapparent carriers, and shedding of leptospires, primarily in the urine, serves as a source of infection for other animals and humans. The clinical signs associated with leptospirosis are variable and depend on the infecting serovar. Leptospira are present in the blood during the first 5 to 10 days after disease onset. Only pathogenic Leptospira species express an outer membrane lipoprotein encoded by the LipL32 gene.

Description:  BactoReal® Kit Leptospira spp. (LipL32) uses real-time PCR for the detection of the LipL32 gene of pathogenic (P1) Leptospira spp. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Leptospira spp. (LipL32)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB00653 50 FAM Cy5 LipL32 gene
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: LipL32 gene of Leptospira spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Leptospira spp. (LipL32) was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit is used for molecular detection of pathogenic Leptospira species targeting the LipL32 gene via real-time PCR.
The LipL32 gene, which is present only in pathogenic strains of Leptospira.
DNA extracted from blood, urine, cerebrospinal fluid, or tissue samples can be used.
Yes, the kit includes an internal control to monitor PCR performance and detect potential inhibitors.
The kit uses real-time PCR with TaqMan® probes for target detection.
The DHUB00653 configuration provides reagents for 50 reactions.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.