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Download All Background: Neisseria meningitidis is a gram-negative bacterium causing meningitis and septicemia. There are 13 clinically significant serogroups, which are classified according to the antigenic structure of their polysaccharide capsule. Five serogroups (A, B, C, Y and W135) are responsible for virtually all cases of the disease. Serogroup B is the predominant one. The capsular transport protein (ctrA) gene is unique to N. meningitidis and can be found in all meningococcal serogroups.
Description: BactoReal® Kit Neisseria meningitidis is a real-time PCR for the qualitative detection of the capsular transport protein (ctrA) gene of all known serogroups of N. meningitidis.Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUB00153 | 50 | FAM | Cy5 | ctrA gene |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: ctrA gene of Neisseria meningitidis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Neisseria meningitidis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).