What is the purpose of the ViroReal® SARS-CoV-2 & SARS assay?

The assay enables the qualitative detection of RNA from SARS-CoV-2, SARS-CoV, and related Sarbecoviruses in environmental samples using one-step RT-PCR.

Which gene target is detected in this assay?

The assay targets a highly conserved region of the N gene, common to all SARS-CoV and SARS-CoV-2 strains, allowing broad detection of Sarbecoviruses.

How does the internal positive control (IPC) function in this assay?

The IPC is an artificial RNA target added during extraction and is detected in the Cy5 channel. It ensures successful RNA extraction and monitors for potential PCR inhibition.

Is the detection with the assay qualitative or quantitative?

The ViroReal® SARS-CoV-2 & SARS assay is intended for qualitative detection only and does not provide quantitative viral load information.

Which PCR instruments are compatible with the assay?

Instruments detecting FAM, VIC, and Cy5 channels such as ABI® 7500, QuantStudio™, MIC, LightCycler® 480 II, and cobas z 480 are compatible.

What is the difference between a ViroReal® Assay and a ViroReal® Kit?

The ViroReal® Assay includes only the primer/probe mix and positive control, and is intended for experienced users. In contrast, ViroReal® Kits include additional components such as an internal control and optimized RT-PCR mix.

ViroReal® Assay SARS-CoV-2 & SARS – Real-Time PCR of SARS-CoV-2 (RdRp) and SARS (Orf1ab)

Name: ViroReal® Assay SARS-CoV-2 & SARS – Real-Time PCR of SARS-CoV-2 (RdRp) and SARS (Orf1ab)
Brand: ingenetix GmbH
SKU: RTGE002
Rating: 5.0 (1 reviews)

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Product Manual ViroReal^® SARS-CoV-2 & SARS	Download PDF
Material Safety Data Sheet ViroReal^® SARS-CoV-2 & SARS	Download PDF
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Background: Coronaviruses are positive single-stranded RNA viruses of the family Coronaviridae. Several different strains of coronaviruses are currently known to infect humans and animals (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV, SARS-CoV-2, NCoV and HCoV-EMC). Strains HCoV-229E, HCoV-NL63, HCoV-OC43, MERS-CoV and HCoV-HKU1 cause cold, upper respiratory infection, bronchiolitis and pneumonia. SARS-CoV, a beta coronavirus, causes the Severe Acute Respiratory Syndrome (SARS). SARS-CoV-2 emerged in Wuhan, China in December 2019 and is responsible for the disease COVID-19 (corona virus disease 2019).

Description: ViroReal^® SARS-CoV-2 & SARS is a test based on one-step reverse transcription real-time PCR. It allows rapid and sensitive detection of SARS-CoV-2 and SARS-CoV RNA purified from environmental samples. The test targets the N gene, allowing universal detection of all so far known SARS-CoV strains including SARS-CoV-2 and SARS-like CoV, without discriminating between the strains.

Product Features:

Amplification and detection: N gene of SARS-CoV-2 and SARS-CoV
Internal Positive Control System to exclude false-negative results
PCR- platforms: runs on all established standard real-time PCR- platforms
Harmonized thermal profiles to run RNA and DNA samples simultaneously
Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal^® Kit SARS-CoV-2 & SARS was validated with the ABI^® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P^® (Agilent), qTOWER³G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler^® 480 I (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (e.g. QuantStudio^™ 5 / 6 / 7 (Thermo Fisher Scientific)).

ViroReal^® SARS-CoV-2 & SARS

Order Number	Reactions	Channel Pathogen	Channel IPC	Target
RTGE002	100	FAM	Cy5	N gene

For research use only
Shipping Temperature: -20 °C dry ice

BactoReal^®, MycoReal^®, ParoReal^® and ViroReal^® Kits run with the same thermal cycling conditions.

ViroReal^® Assay SARS-CoV-2 and SARS

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