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| Product Manual ViroReal® Kit SARS-CoV-2 & SARS |
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| Material Safety Data Sheet ViroReal® Kit SARS-CoV-2 & SARS |
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Background: Coronaviruses are positive single-stranded RNA viruses of the family Coronaviridae. Several different strains of coronaviruses are currently known to infect humans and animals (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV, SARS-CoV-2, NCoV and HCoV-EMC). Strains HCoV-229E, HCoV-NL63, HCoV-OC43, MERS-CoV and HCoV-HKU1 cause cold, upper respiratory infection, bronchiolitis and pneumonia. SARS-CoV, a beta coronavirus, causes the Severe Acute Respiratory Syndrome (SARS). SARS-CoV-2 emerged in Wuhan, China in December 2019 and is responsible for the disease COVID-19 (corona virus disease 2019).
Description: ViroReal® Kit SARS-CoV-2 & SARS is a real-time PCR for the qualitative detection of the of RNA of the nucleocapsid protein gene (N gene) of SARS-CoV-2, SARS-CoV and SARS-related coronavirus, purified from environmental samples. The test allows universal detection of all so far known SARS-CoV strains including SARS-CoV-2 and SARS-related CoV, without discriminating between the strains. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG). This Kit was formerly available as order nr. DHUV02313.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DENV00113 | 100 | FAM | Cy5 | N gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: N gene of SARS-CoV-2 and SARS-CoV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit SARS-CoV-2 & SARS was validated with the ABI® 7500 Fast Real-Time PCR System (fast
cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent),
qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas
Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and
differentiate fluorescence in FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).