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| Product Manual ViroReal® Kit SARS Coronavirus & Influenza A/B |
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| Material Safety Data Sheet ViroReal® Kit SARS Coronavirus & Influenza A/B |
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Background:
Coronaviruses are positive single-stranded RNA viruses of the family Coronaviridae. Several different strains of coronaviruses are currently known to infect humans and animals (HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, MERS-CoV, SARS-CoV, SARS-CoV-2, NCoV and HCoV-EMC). Strains HCoV-229E, HCoV-NL63, HCoV-OC43, MERS-CoV and HCoV-HKU1 cause cold, upper respiratory infection, bronchiolitis and pneumonia. SARS-CoV, a beta coronavirus, causes the Severe Acute Respiratory Syndrome (SARS). SARS-CoV-2 emerged in Wuhan, China in December 2019 and is responsible for the disease COVID-19 (corona virus disease 2019).
Influenza is an acute infectious disease caused by influenza virus A, B or, to a much lesser extent, influenza virus C. Influenza viruses are enveloped viruses with single-stranded, segmented RNA with negative polarity as genome. These viruses can be found worldwide. Epidemics and pandemics are mainly caused by influenza virus A, due to antigenic drift of the hemagglutinin and neuraminidase molecules. Type B and C influenza viruses are isolated almost exclusively from humans, while influenza A viruses infect a wide variety of warm-blooded animals.
Description: ViroReal® Kit SARS Coronavirus & Influenza A/B is a test, based on one-step reverse transcription real-time PCR, for the detection of the N gene of SARS-CoV-2, SARS-CoV and SARS-related coronavirus (Sarbecovirus), of the matrix protein gene of influenza A virus and of the hemagglutinin gene of influenza B virus. The test is intended to analyze environmental samples.
This Kits was formerly available as order nr. DHUV02513.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DENV00313 | 100 | FAM, VIC | Cy5 | N gene & hemagglutinin/matrix protein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: N gene of SARS-CoV and hemagglutinin/matrix protein gene of influenza A/B virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)
PCR platforms: ViroReal® Kit SARS Coronavirus & Influenza A/B was validated with the Applied Biosystems® 7500 instrument (Thermo Fisher Scientific) and was also tested with LightCycler® 480 II (Roche Diagnostics), MIC instrument (bio molecular systems) and Mx3005P® QPCR System (Agilent). It is also compatible with other real-time PCR instruments detecting and differentiating fluorescence in FAM, VIC and Cy5 channel (e.g. cobas z 480 Analyzer (Roche), QuantStudio™ 5 and QuantStudio™ 7 Pro (Thermo Fisher Scientific) and qTOWER3G (Analytik Jena)).